
KRIOPRESERVASI SEMEN
Kriopreservasi semen merupakan proses penurunan suhu, dehidrasi sel, pembekuan, penyimpanan dan kemudian pencairan kembali. Kriopreserasi semen memegang peranan penting dalam pelaksanaan inseminasi buatan karena dapat mengakselerasi penyebaran keragaman genetik dan memfasilitasi distribusi superior genetik keseluruh wilayah. Proses kriopreservasi semen yang baik akan meningkatkan efisiensi dan tingkat kesuksesan dalam program inseminasi buatan (Ugur et al., 2019) Program inseminasi buatan sepenuhnya bisa dilaksanakan hanya dengan semen beku yang memungkinkan penyimpanan yang lebih lama dan cakupan distribusi yang tidak terbatas (Layek et al., 2016). Manfaat kriopreservasi semen diantaraya: efisiensi penggunaan pejantan, inseminasi buatan tidak dibatasi waktu dan tempat, memudahkan transportasi, lebih ekonomis, dan mempunyai daya simpan yang panjang.
Semen beku merupakan semen yang telah mengalami proses pengenceran dengan bahan pengencer kemudian ditambahkan glicerol dalam proses pembekuannya. Pembekuan semen dapat dilakukan secara bertahap atau langsung (vitrifikasi), pembekuan semen dengan menggunakan medium nitrogen cair (N2 cair) dengan suhu -196oC. Spermatozoa yang telah dibekukan akan mampu bertahan hidup dalam kurun waktu yang lama bahkan setelah 30 tahun baru akan mengalami penurunan kualitasnya (Ismaya, 2014). Proses kriopreservasi tersebut berakibat pada kerusakan pada spermatozoa sehingga berdampak pada fertilitas. Layek et al. (2016) mengungkapkan bahwa kerusakan akibat kriopreservasi bisa mencapai 50%. Kerusakan spermatozoa dapat berupa kerusakan membran plasma, membran akrosom, dan kerusakan biokimia dalam sel seperti penurunan aktivitas proteolik akrosom (Ismaya, 2014). Beberapa penyebab kerusakan spermatozoa saat proses pembekuan diantaranya cekaman dingin, dan terbentuknya kristal es (Watson, 1995; Watson, 2000; Susilawati, 2011).
Cekaman Dingin
Cekaman dingin atau Cold shock merupakan penyebab utama penurunan kualitas semen (Ugur, 2019). Spermatozoa kehilangan kekuatan membran dan fungsinya ketika terjadi penurunan suhu 0oC -20oC. Fenomena tersebut terjadi karena terjadi perubahan fase membran lipid sehingga membran lipid kehilangan kemampuan permeabilitas. Cekaman dingin diketahui bedampak pada penurunan kemampuan kinetik ekor spermatozoa sehingga terjadi penurunan motilitas (Watson, 1995).
Penambahan pengencer yang berisi krioprotektan sebelum proses pembekuan dapat mengurangi resiko penurunan kualitas semen akibat dari cold shock. Saat ini, pengencer yang berbasis kuning telur paling umum digunakan dalam proses kriopreservasi berbagai macam ternak. Kandungan Low Density Lipoprotein (LDL) dapat melindungi spermatozoa dari penurunan kualitas semen seperti motilitas, kekuatan membran, dan kemampuan fertilisasi (Bencharif et al., 2010).
Pembentukan Krista Es dan Cekaman Osmotik
Penurunan suhu melewati 0oC larutan ekstraseluler dan intraseluler membentuk kristal es, sehingga dapat menyebabkan kerusakan seara mekanis terhadap membran plasma (Watson, 1995). Waktu pembekuan yang tepat memegang peranan penting dalam meminimalisir kerusakan sel spermatozoa. Pembekuan lambat meminimalisir kerusakan mekanis yang disebabkan oleh kristal es. Namun, kerusakan terjadi akibat efek padatan yang diakibatkan terjadinya perbedaan tekanan osmotik sehingga fase cairan dalam sel keluar menyisakan fase padatan dalam sel. Sebaliknya, pembekuan cepat mencegah kerusakan sel spermatozoa akibat dari efek padatan namun tetap terjadi kerusakan mekanis akibat dari pembentukan kristal es berukuran besar (Hafez, 2000).
Penambahan krioprotektan (gliserol) dapat menurunkan resiko kerusakan spermatozoa. Penambahan krioprotektan akan menurunkan titik beku sel spermatozoa hingga -196oC. Mekanisme perubahan titik beku disebabkan masuknya krioprotektan ke dalam sel karena sifatnya yang higroskopis, kemudian menggantikan cairan dalam sel dengan cairan krioprotektan tersebut sehingga dapat meminimalisir kerusakan (Susilawati, 2011).
Ketahanan spermatozoa terhadap proses pembekuan dapat dipengaruhi oleh berbagai faktor seperti efek dari temperatur dan pengaruh musim (Kiovisto, 2016). Badan Standarisasi Nasional (2017) mensyaratkan bahwa semen beku yang layak digunakan memiliki motilitas lebih dari atau sama dengan 40%.
DAFTAR PUSTAKA
Bencharif, D., L. Amirat-Briand, A. Garand, M. Anton, E. Schmitt, S. Desherces, G. Delhomme, M.L. Langlois, P. Barrière dan S. Destrumelle, 2010. Freezing Canine Sperm: Comparison of Semen Extenders Containing
Hafez E.S.E. 2000. Preservation and Cryopreservation of Gametes and Embryos. In. Hafez E.S.E (ed). Reproduction in Farm Animals 7th Ed. Lippincott Williams & Wilkins, USA.
Ismaya. 2014. Bioteknologi Inseminasi Buatan pada Sapid dan Kerbau. Gadjah Mada University Press, Yogyakarta.
Kiovisto, MB, MTA Costa, SHV Perri and WRR Vicente. 2008.The Effect of Season on Semen Characteristics and Freezability in Bos indicus and Bos taurus Bulls in the Southeastern Region of Brazil. Reprod. Dom. Anim. 44: 587–592 .
Layek, S.S., T.K. Mohanty , A. Kumaresan , J.E. Parks . 2016. Cryopreservation of Bull Semen: Evolution from Egg Yolk Based to Soybean Based Extenders. Anim. Reprod. Sci. 172 : 1–9.
Susilawati, T.. 2011. Spermatology. UB Press, Malang.
Ugur M. R., A. S. Abdelrahman, H C. Evans, A. A. Gilmore, M. Hitit, L. A. Arifiantini, B. Purwantara, A. Kaya, dan E. Memili. 2019. Advances In Cryopreservation Of Bull Sperm. Front. Vet. Sci. 6:268.
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Appearances– the shade/color of the composite fillings can be very closely matched to the shade of existing teeth. Compounds are particularly well matched for usage in front teeth or visible parts of teeth. Destruction of more tooth structure– healthy parts of the tooth have to frequently be gotten rid of to make a space huge sufficient to hold the amalgam dental filling. Poor visual appeals– silver fillings do not match the color of natural teeth.
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Compound fillings, additionally called tooth-colored or white dental fillings, are made from a composite resin material that is matched to the shade of your natural teeth. They are a prominent choice for recovering corroded or broken teeth in areas that reveal when you smile or talk. Ceramic fillings, additionally known as porcelain fillings, are constructed from a tooth-colored product that blends in with the all-natural teeth. They are long lasting and resilient, making them a preferred selection for back teeth. Nonetheless, they may be a lot more pricey than composite fillings. Front teeth dental caries are dealt with similarly as any kind of other dental caries.
Age-related stainJust like any kind of various other body part, teeth can deteriorate and tarnish gradually. These stains are brought on by the dimming of the dentin, which is revealed due to the wearing down of the enamel, with age. This is a natural aging procedure, so do not stress on your own by assuming that it is your fault that you do neglect your teeth or have a poor eating habit. There are several things you can do to prevent tooth spots, prior to or after teeth-whitening. Tooth polishing done throughout teeth cleansings by using a motorized tool.
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But also today, there are still grownups with tetracycline teeth discoloring. If you stumble upon someone with the problem, it probably means they were suggested the antibiotic when they were more youthful than 8 years old. Gradually, as teeth discoloring was found in people taking this medication, researches discovered that tetracycline was triggering the irreversible staining of teeth. Fortunately is, there are strategies that can be made use of to bleach tetracycline teeth, however often, the problem of the teeth may be beyond lightening. Making use of an OTC lightening item every once in a while can go a lengthy means in stopping your teeth from staining. Cancer treatments.Chemotherapyespecially head and neck radiation can cause tooth staining.
This condition is fast-acting and can be harmful if not treated. RA is an autoimmune condition that causes persistent, uncomfortable inflammation in joints throughout the body, consisting of the temporomandibular joint. © 2023 Healthline Media UK Ltd, Brighton, UK. All rights reserved. Teeth grinding can cause pain and other difficulties, however a mouthguard can assist shield against it.
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Utilize your fingers and palms to massage therapy the sides of your head, temple, and jaw to make it simpler to flow blood. Attempt to select the same time to visit sleep and wake up everyday. 5) Gingivitis- Gum troubles in its beginning are called gingivitis.
As soon as diagnosis is validated, activity can be taken, such as wearing an evening guard for teeth grinding or mouth insulation, to lower any oral damage and determine the underlying cause. An additional usual manner in which bruxism is identified is throughout an oral exam is that the dentist checks the teeth for evidence of grinding upon a routine exam. If the initial signs of bruxism are noted, the dental practitioner might take some time to examine whether the signs and symptoms are dynamic and establish if therapy is called for. This mouthguard attributes a density of 3 mm to maximize its effectiveness against extreme teeth grinding or jaw clenching during rest.
Finally, your dental expert will form the filling product to guarantee a proper bite and appearance. They might additionally polish the filling up to make it smooth and shiny. Your dentist will certainly start by numbing the location around your tooth with a local anesthetic. This will aid make certain that you really feel little to no pain during the treatment. They assist protect the tooth enamel and avoid further damage from different sorts of dental caries.
An individual ought to take the time to research study and pick one that does the most effective job of satisfying their requirements and the form of their mouth. These are one-size-fits-all, disposable guards that safeguard the back teeth from light-to-moderate grinding. TMJ, a problem of the temporomandibular joint, can create pain and discomfort.
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All in all, just see to it that you look for consultation with a dental expert or physician before trying botox. A consultation with an oral specialist ought to be your first step in validating medical diagnosis and evaluating the extent of any type of damages created. The teeth are totally covered by the mouth guard so there is no get in touch with between any kind of upper and reduced teeth. Muscle discomfort around the jaw and the temporomandibular joint.
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Oil of Oregano to Quit Decay.Establish a Healthy pH Degree with Xylitol.Clove Oil for Discomfort Relief and Prevention.Neem Bark to combat Infection.Green Tea as an All-natural Fluoride Treatment.Keep Teeth Healthy with Licorice Root.Diet Changes to Decrease Germs.
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Our website is not intended to be an alternative to specialist clinical advice, diagnosis, or therapy. It takes the eyes of an oral specialist to figure out if there is a much more severe trouble. Only if the dental practitioner’s assessment is delayed, the result will be more major.
You may discover this especially when having hot or cool foods or beverages. By complying with these ideas for exactly how to stop tooth decay from spreading, you’ll find that you can keep your child’s teeth in great problem for many years to find. If origin canal treatment isn’t possible, your healthcare provider might recommend tooth removal. You may need an oral bridge or oral implant to replace a pulled permanent tooth.
Utilize the floss threader to get under the cable and in between the following set of teeth. Guide the pointed end of the floss threader under the braces cable and draw the floss through till it can be gripped with both hands. Floss backwards and forwards the side of one tooth, getting under the gumline, and afterwards up and down the side of the various other tooth.
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Yet in situations of crack, substantial decay, or malformation of primary teeth, dental experts commonly choose stainless steel or ceramic crowns. Crowns keep the tooth while stopping the degeneration from dispersing. New products indicate pediatric dental experts have more dental filling and fixing options than ever before. A silver-colored product called amalgam was when the compound of option for most fillings in long-term teeth. Now, various other products like composite resins are coming to be prominent. Materials bond to the teeth so the filling will not bulge, and likewise can be made use of to rebuild teeth harmed with injury or problems like a slit palate.
One simple means of making brushing fun for children is allowing them select their very own toothbrush. There are lots of vivid ones aimed at youngsters, such as Colgate Barbie and Colgate Spiderman. These are decorated with cartoon personalities, and have extra-soft bristles and a smaller head. Having a toothbrush they like will only make your youngsters anticipate brushing their teeth, which’s a valuable routine to have.
Moms and dads, nonetheless, need to still comply with up and brush them again to see to it they’re clean. It is known to lower tooth cavities in primary teeth and grown-up teeth. It likewise aids make teeth solid by setting the tooth enamel. Water filters, such as Brita, do not eliminate fluoride and are fine to use.
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Preventing baby container tooth decay– likewise called early childhood years dental caries– is one of the most essential infant tooth treatment concerns. This condition occurs when the baby sleeps with milk, formula, or juice in their mouth. The sugars swimming pool inside the mouth and can lead to tooth cavities in both the top and bottom teeth. Motivate kids to drink more water to aid prevent tooth decay.
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